Descripció del projecte
Infertility is a global disease of high prevalence and far reaching personal and social consequences. On average, 1 in 9 couples trying to conceive will
have some difficulties and most will need assisted reproductive technologies (ART). Despite numerous technical advances, ART live birth rates are
30-35% worldwide, suggesting ART cycles remain suboptimal.
Embryonic implantation in the maternal uterus is a poorly characterized process by which the embryo trophectoderm makes contact with the
endometrium. After an initial mutual recognition between the two compartments, a complex molecular crosstalk between them coordinates
embryo embedding and nidation. While classically implantation was considered mainly driven by the embryo, with the endometrium playing a more
passive role, recent research has shown that the endometrium and its receptivity is a crucial and active player in the process. Clinically, aproximately
4% of women suffer from repeated implantation failure (RIF), characterized by recurrent inability of the endometrium to accept good quality embryos.
The main objective of this industrial project is to develop and validate an in vitro model of human uterine RIF, in order to identify biomarkers of RIF.
The specfic objectives are:
1) Establish an in vitro culture model using decidualized stromal cells from normal and RIF patients.
2) Characterize the transcriptomic and proteomic profiles of each compartment during the process of attachment.
3) To identify secreted endometrial proteins.
The developed model will allow us to establish a human in vitro model for RIF for further research of this casue of infertility and the development of
diagnostic tools and clinical interventions.
This project will
